Proliferation and differentiation are controlled through chromatin remodelling. Therefore, there is an enormous biological significance and clinical value in understanding how specific signalling pathways are affected by histone replacement in the nucleosome. In this work, mass spectrometry was used to screen HC11 mammary epithelial cells for changes in histone levels throughout cell differentiation. The canonical histone isoform Histone H2A type 2-C (Hist2h2ac) was found only in undifferentiated/proliferating cells.
Recently it was published in Histopathology a work developed by the Department of Pathology of the Ipatimup Diagnostics in collaboration with Hospital Pedro Hispano and Hospital Prof. Doctor Fernando Fonseca concerning the minimum number of neoplastic cells needed to quantify robustly the amplification of the HER2 gene in breast cancer.
Recentemente foi publicado no Histopathology um trabalho desenvolvido pelo Laboratório de Anatomia Patológica do Ipatimup Diagnósticos em colaboração com os serviços de Anatomia Patológica do Hospital Pedro Hispano e do Hospital Prof. Doutor Fernando Fonseca sobre o número mínimo de células neoplásicas necessárias para quantificar de forma reprodutível a amplificação do gene HER2 em cancro da mama.
