Catarina Brito

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A novel in vitro Ewing's Sarcoma 3D cell model

Autores e Afiliações:

Giacomo Domenici 1,2 Rodrigo Eduardo 1,2 Helena Castillo-Ecija 3 Gorka Orive 4,5 Ángel Montero Carcaboso 3 Catarina Brito 1,2

1-iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal

2-Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal

A novel in vitro Ewing's Sarcoma 3D cell model

Ewing’s Sarcoma (ES) is the second most frequent bone tumour in children and young adults, with very aggressive behaviour and significant disease recurrence. To better study the disease and find new therapies, experimental models are needed. In this work, we developed a novel ES cell culture method based on 3D spheroids. Our culture method maintains cell viability and ES intrinsic characteristics preservation for at least one month, allowing ex-vivo testing of anticancer drugs.

 

Patient-derived ovarian cancer explants: preserved viability and histopathologic

Ovarian carcinoma remains a major therapeutic challenge due to its tendency to develop resistance after initial response to chemotherapy. In this work, a collaboration between iBET, AbbVie and IPOLFG, we developed ovarian carcinoma patient-derived explant (OvC-PDE) cultures that retained architecture and cell type heterogeneity of the original tumour. This patient-derived model have potential applications in the study of drug response and resistance mechanisms and in the development of innovative precision medicine approaches.

Patient-derived ovarian cancer explants: preserved viability and histopathologic

Ovarian carcinoma remains a major therapeutic challenge due to its tendency to develop resistance after initial response to chemotherapy. In this work, a collaboration between iBET, AbbVie and IPOLFG, we developed ovarian carcinoma patient-derived explant (OvC-PDE) cultures that retained architecture and cell type heterogeneity of the original tumour. This patient-derived model have potential applications in the study of drug response and resistance mechanisms and in the development of innovative precision medicine approaches.

A novel culture method to study breast cancer biology, in particular ERα signaling

The main therapeutic strategy used in 75% of all breast cancers targets estrogen receptor α (ERα). However, the mechanisms underlying ERα therapeutic resistance are still elusive hampered by the challenges in developing suitable experimental models. In this work, a novel ex-vivo culture strategy was developed. This strategy is based on tissue microstructures encapsulation in alginate, enabling maintenance of original tissue structure, cell populations and extracellular matrix, thus recapitulating the tumoral microenvironment in which ERα signaling is sustained.

Novo método de cultura para o estudo do cancro da mama

A principal estratégia terapêutica utilizada em 75% dos cancros da mama tem como alvo o recetor de estrogénio alfa (ERα). Em alguns casos, contudo, esta terapêutica mostra-se ineficaz sendo que os mecanismos associados a esta resistência são ainda pouco conhecidos.  Neste trabalho, os investigadores desenvolveram uma nova estratégia de cultura de tecido tumoral que permite a manutenção da estrutura original dos tecidos, incluindo populações celulares e matriz extracelular.