The therapeutic landscape in breast cancer has benefited from the development of variate antibody-based therapies. Yet, pre-clinical models capable of recapitulating the complex crosstalk within the tumor microenvironment (TME) are needed to properly address the effect of such therapies. In this work, we devised cell models able to accommodate different cell populations (3D-3), by adding stromal and immune components to multicellular tumor spheroids. The models were microencapsulated in alginate and kept under stirred conditions, to recreate critical hallmarks of the TME.
O tratamento de cancro de mama tem beneficiado do desenvolvimento de várias terapias à base de anticorpos. Contudo, para avaliar corretamente o efeito destas terapias são necessários modelos pré-clínicos capazes de representar as complexas interações que correm dentro do microambiente tumoral (TME). Neste trabalho, desenvolvemos modelos celulares capazes de acomodar diferentes tipos de células (3D-3), ao adicionar uma componente estromal e imunitária a esferoides tumorais multicelulares.
Ewing’s Sarcoma (ES) is the second most frequent bone tumour in children and young adults, with very aggressive behaviour and significant disease recurrence. To better study the disease and find new therapies, experimental models are needed. In this work, we developed a novel ES cell culture method based on 3D spheroids. Our culture method maintains cell viability and ES intrinsic characteristics preservation for at least one month, allowing ex-vivo testing of anticancer drugs.
