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A novel in vitro Ewing's Sarcoma 3D cell model

Autores e Afiliações:

Giacomo Domenici 1,2 Rodrigo Eduardo 1,2 Helena Castillo-Ecija 3 Gorka Orive 4,5 Ángel Montero Carcaboso 3 Catarina Brito 1,2

1-iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal

2-Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal

A novel in vitro Ewing's Sarcoma 3D cell model

Ewing’s Sarcoma (ES) is the second most frequent bone tumour in children and young adults, with very aggressive behaviour and significant disease recurrence. To better study the disease and find new therapies, experimental models are needed. In this work, we developed a novel ES cell culture method based on 3D spheroids. Our culture method maintains cell viability and ES intrinsic characteristics preservation for at least one month, allowing ex-vivo testing of anticancer drugs.