breast cancer

SQUARE Brussels Meeting Centre, Brussels, Belgium

07 - 09 May, 2015

See more informations here: http://www.esmo.org/Conferences/IMPAKT-2015-Breast-Cancer

SQUARE Brussels Meeting Centre, Brussels, Belgium

07 - 09 May, 2015

See more informations here: http://www.esmo.org/Conferences/IMPAKT-2015-Breast-Cancer

Authors and Affiliations:

Sonia A. Melo^{1, 2}, Hikaru Sugimoto^{1, 2}, Joyce T. O’Connell², Noritoshi Kato², Alberto Villanueva³, August Vidal⁴, Le Qiu⁵, Edward Vitkin⁵, Lev T. Perelman⁵, Carlos A. Melo^{6, 7}, Anthony Lucci⁸, Cristina Ivan⁹, George A. Calin¹⁰, Raghu Kalluri^{1, 2,}

An electrochemical immunosensor for the analysis of HER2 ECD in human serum samples was developed. To achieve this goal a screen-printed carbon electrode, modified with gold nanoparticles, was used as transducer surface. A sandwich immunoassay, using two monoclonal antibodies, was employed and the detection of the antibody-antigen interaction was performed through the analysis of an enzymatic reaction product by linear sweep voltammetry. The calibration curve was established between 15 and 100 ng/mL and a limit of detection of 4.4 ng/mL was achieved.

Authors and Affiliations:

M. Luísa Dória*¹, Ana S. Ribeiro*¹, Jun Wang², Cândida Z. Cotrim¹, Pedro Domingues¹, Cecilia Williams², M. Rosário Domingues¹ and Luisa A. Helguero¹

¹Mass Spectrometry Centre, Organic Chemistry and Natural Products Research Unit, Department of Chemistry, Universidade de Aveiro, Campus de Santiago, Aveiro, Portugal; and

Researchers at IPATIMUP (Joana Paredes group) are focused in P-cadherin, an adhesion molecule that induces cancer stem cell and invasive properties in breast cancer. In its newest publication, this group found a downstream signaling pathway that helps clarify P-cadherin mediated aggressive effects.

This work reports an important crosstalk between P-cadherin and the laminin receptor α6β4, a link that was never previously described. The activation of this heterodimer points to a new molecular mechanism that may be targeted to counteract the effects induced by this adhesion molecule.

Authors and Affiliations:

Filipa Morais-Santos^1,2, Vera Miranda-Gonçalves^1,2, Sílvia Pinheiro^1,2, André F Vieira³, Joana Paredes³, Fernando C Schmitt^3,4,5,6, Fátima Baltazar^1,2,* and Céline Pinheiro^1,2,7,*

¹ School of Health Sciences, Life and Health Sciences Research Institute (ICVS), University of Minho, Campus of Gualtar, Braga, Portugal

² ICVS/3B’s – PT Government Associate Laboratory, Braga, Guimarães, Portugal