Associação Portuguesa de Investigação em Cancro
Vírus do papiloma humano em carcinoma da conjuntiva: um estudo em Moçambique
Vírus do papiloma humano em carcinoma da conjuntiva: um estudo em Moçambique
A incidência do carcinoma da conjuntiva tem aumentado em Africa e dados do Hospital Central de Maputo em Moçambique registam um aumento muito significativo no período de 2006-2008, em comparação com 1991-1993, de 4 e 18x, respectivamente em homens e mulheres. Sabe-se que estão associados a esta neoplasia a radiação ultravioleta, a infecção por HIV e, de forma ainda pouco esclarecida, a infecção por HPV. O nosso estudo pretendeu avaliar se havia, em neoplasias da conjuntiva, presença de vírus HPV e, em caso afirmativo, que tipos de vírus. O que observámos foi uma elevada presença de vírus e, curiosamente, de tipos de HPV que são geralmente cutaneotrópicos e não-oncogénicos. A interacção com infecção por HIV pode ser responsável por esta associação inesperada.
Carrilho C (a), Gouveia P (c), Yokohama H (a), Lopes JM (c,d), Lunet N (e,f), Ferro J (b), Ismail M (a), Walboomers J (g), Sobrinho-Simões M (c,d), David L (c,d)
(a) Department of Pathology, Faculty of Medicine of the Eduardo Mondlane University and Maputo Central Hospital, Maputo (b) Department of Pathology, Beira Central Hospital, Beira, Mozambique (c) IPATIMUP - Institute of Molecular Pathology and Immunology (d) Department of Pathology, Faculty of Medicine (e) Department of Clinical Epidemiology, Predictive Medicine and Public Health, Faculty of Medicine of the University of Porto (f) Institute of Public Health of the University of Porto (ISPUP), Porto, Portugal (g) Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
Resumo:
The infection with human papillomavirus (HPV) has been described as a risk factor for squamous cell carcinoma of the conjunctiva (SCCC), although the evidence is conflicting. To assess the relation between HPV infection and intraepithelial neoplasia or SCCC, we evaluated archived material from biopsies of the conjunctiva performed at the Maputo Central Hospital (Mozambique) in patients with suspected eye cancer. The quality of DNA was assessed by PCR using [beta]-globin-specific primers. A total of 22 consecutive biopsies (intraepithelial neoplasia, SCCC, and benign conditions) positive for [beta]-globin were further tested for HPV infection by PCR using the general primers GP5+/GP6+ and CPI/CPII. In addition, PCR with type-specific primers HPV 16 and HPV 18 was performed. Nineteen biopsies corresponded to intraepithelial neoplasia (two low-grade and nine high-grade) or SCCC (n=8), from which 11 (57.9%) tested positive for HPV infection; nine were positive for CPI/CPII, including one case also positive for GP5+/GP6+ and HPV 18, and the remaining two tested positive only for HPV 16. HPV DNA was not detected in any of the three biopsies of benign conditions. These results suggest a stronger association between infection with cutaneous HPV and SCCC than for mucosal HPV. However, further research is required to clarify the relation between HPV and SCCC as well as to understand the potential of the HPV vaccine currently available for cervical cancer to prevent SCCC.
Revista:
European Journal of Cancer Prevention
