Considerable attempts have been made to produce adequate matrices or scaffolds that mimic bone ECM for applications in tissue engineering and regenerative medicine. In this context, several factors must be considered, such as the modification of biomaterial surfaces using growth factors, living bone cells or proteins, to guide cellular responses in bone remodeling, like osteoblast adhesion and long term functionality expressed as proliferation, synthesis of alkaline phosphatase and deposition of calcium containing mineral.
