ovarian cancer

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IMM researchers identify new immune mechanism that promotes ovarian cancer growth

A team of researchers from Instituto de Medicina Molecular (IMM) has uncovered an unexpected cellular cross-talk behind ovarian cancer growth that can be exploited as a novel therapeutic target for future immuno-oncology strategies. The work reveals that gamma delta (γδ) T lymphocytes, which are normally seen as anti-tumour, can, surprisingly, promote tumour growth in ovarian cancer. The research, led by Prof. Bruno Silva-Santos, has now been published in PNAS (Proceedings of the Natural Academy of Sciences of the USA).

Characterization of Binding Epitopes of CA125 Monoclonal Antibodies

The most used cancer serum biomarker is the CA125 immunoassay for ovarian cancer that detects the mucin MUC16. However, the monoclonal antibodies (OC125 and M11) used in the serum assay have epitopes that remain elusive. In this study, we demonstrate for the first time that neither N- nor O-glycosylation appear to substantially influence binding of OC125 and M11 mAbs and narrowed down the epitopes to a segment containing parts of two consecutive SEA domains with a linker.

Characterization of Binding Epitopes of CA125 Monoclonal Antibodies

The most used cancer serum biomarker is the CA125 immunoassay for ovarian cancer that detects the mucin MUC16. However, the monoclonal antibodies (OC125 and M11) used in the serum assay have epitopes that remain elusive. In this study, we demonstrate for the first time that neither N- nor O-glycosylation appear to substantially influence binding of OC125 and M11 mAbs and narrowed down the epitopes to a segment containing parts of two consecutive SEA domains with a linker.

Glycosylation of exosomes from ovarian carcinoma cells

Authors and Affiliations:

Cristina Escrevente1, Nicolas Grammel2, Sebastian Kandzia2, Johannes Zeiser2, Erin M. Tranfield3, Harald S. Conradt2, Júlia Costa1

1.Laboratory of Glycobiology, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal

2.GlycoThera GmbH, Hannover, Germany

3.Electron Microscopy Facility, Instituto Gulbenkian de Ciência, Oeiras, Portugal

 

Abstract: